Adnavance Technologies
Adnavance is aggressively developing, optimizing and integrating key technologies that are needed to rapidly deliver revolutionary diagnostics products – products that are differentiated by superior simplicity, quality and speed.
At the Core
Our patented metalized-DNA (M-DNA™) platform is based on the conducting properties of hybridized DNA. Under strict and patented reaction conditions, certain metal ions can enter the central core of hybridized DNA and displace the hydrogen bonds, greatly enhancing the conductive properties of DNA. The result is a new confirmation of DNA, metalized-DNA (M-DNA™) that is highly conductive. The company uses a microarray of micron-sized electrodes to detect M-DNA™ and the inherent ultra-sensitivity arises from the differential measurement in conductivity between hybridized DNA (B-DNA) and metalized-DNA (M-DNA™). This change in conductivity is so large that it may eliminate the need for amplification used in current molecular-based tests.
Microarray: Integrated Functionality
At the heart of our detector is a microarray chip containing 32 electrodes (1 to 10 microns in diameter) and capable of simultaneous detection of up to 32 target molecules. Many technical issues were solved within the first three generations of this chip. Our fourth-generation chip is fabricated using standard semiconductor methods at a commercial high-throughput foundry. The chip is fully optimized through standard industry practices including DOE (Design of Experiments).
Optical Image of the microarray
SEM images of the microelectrodes
Fluidics: Rapid Delivery
Liquid samples and processing buffers are delivered to our biochip using both macrofluidic and microfluidic conduits. Our embedded system controls the flow of multiple streams and aliquots. Our microfluidic chips utilize a simple coupling mechanism to mate with the microarray sensors at desired pressures and without leaks. The microfluidic chips are rapidly prototyped using PDMS (polydimethylsiloxane)-based technologies.
Device: Simultaneous Readout
We are completing a pre-prototype instrument to demonstrate the M-DNA™ technology on biological samples. Our embedded system utilizes the latest in analog and digital signal processing to deliver a truly simultaneous potentiostat readout of up to 36 channels. Large data streams are handled through our firmware and software.
